SCREENING AND CHARACTERIZATION OF ENDOPOLYSACCHARIDE FROM Pleurotus pulmonarius IN SUBMERGED CULTURE FERMENTATION

  • Shaiful Azuar Mohamad
  • Nurul Shahnadz Amir Hamzah
  • Choong Yew Keong
  • Mohd Yusof Hamzah
  • Khairuddin Abdul Rahim
  • Wan Mohtar Wan Yusoff

Abstract

The fermentation of Pleurotus pulmonarious (non-irradiated) and Pleurotus pulmonarious (irradiated) were carried out in Erlenmeyer flask 500 mL (working volume 250 mL) and screened for the presence of β-glucan (1, 3:1, 6). The biomass obtained was extracted using Modified Mizuno Method to get the endopolysaccharide. The endopolysaccharides of Pleurotus pulmonarious (irradiated) contained higher content of β-glucan (1, 3:1, 6) with 16.7 g/100g crude polysaccharide compared to the non-irradiated strain. The irradiated strain was chosen for further investigation. Fractionation of endopolysaccharide Pleurotus pulmonarious (irradiated) using column chromatograph yielded 7 fractions. The first fraction (F1) contained high molecular weight fraction ~ 105 Da which potential immunomodulation characteristics. The Pleurotus pulmonarious (irradiated) was produced in a 5 L air-lift bioreactor. The highest biomass was obtained at air flowrate of 2 L/min, yielding productivity of 2.56 g/L.d.

Published
2017-07-11
How to Cite
MOHAMAD, Shaiful Azuar et al. SCREENING AND CHARACTERIZATION OF ENDOPOLYSACCHARIDE FROM Pleurotus pulmonarius IN SUBMERGED CULTURE FERMENTATION. Jurnal Sains Nuklear Malaysia, [S.l.], v. 29, n. 2, p. 52-59, july 2017. ISSN 2232-0946. Available at: <https://jsnm.nuclearmalaysia.gov.my/index.php/jsnm/article/view/117>. Date accessed: 25 jan. 2020.