DETERMINATION OF MUSHROOM MONOSACCHARIDES BY LIQUID CHROMATOGRAPHY MASS-SPECTROMETRY (LCMS) AND MACROPHAGE ACTIVATION BY POLYSACCHARIDES FROM Polyporus umbellatus, Fuscoporia obliqua, Cordyceps militaris AND Pleurotus ostreatus
The aims of this study are to determine the monosaccharide composition in polysaccharide and macrophage activation in cell culture by purified polysaccharide. In this study, mushroom polysaccharide was obtained from mycelia of Polyporus umbellatus, Fuscoporia obliqua, Cordyceps militaris and Pleurotus ostreatus by hot water extraction and ethanol precipitation. Polysaccharide was hydrolyzed by Trifluacetic Acid (TFA) to get monosaccharides such as fructose, glucose, mannose and xylose. The detection of monosaccharides by Liquid Liquid Liquid Liquid Chromatography MassChromatography Mass Chromatography Mass Chromatography MassChromatography MassChromatography Mass -Spectrometry (Spectrometry ( Spectrometry (Spectrometry ( Spectrometry ( Spectrometry ( Spectrometry (Spectrometry (LCMS) shown that fructose, glucose, mannose and xylose were only present in P. umbellatus. Glucose detected in P. ostreatus was 96.1 %, the highest compared to other species. For macrophage activation analysis, Nitric Oxide (NO) in macrophage cell culture added with polysaccharide extract from P. umbellatus was highest as compared to others mushroom species samples. Macrophage cell could be activated by mushroom polysaccharide and then produced NO due to antitumor activity which is harmful to cancer cells. Hence, mushroom polysaccharide has a high potential in medicinal use aspects, especially related to cancer cell study. Therefore mushroom species or strain with high in polysaccharide content is potentially beneficial for medicinal purposes. However, details studied have to be conducted for further investigation.