RADIATION-INDUCED BYSTANDER EFFECTS (RIBE) IN DBTRG-05MG HUMAN GLIOBLASTOMA CELLS
This study aims to investigate the radiation-induced bystander effects (RIBE) in DBTRG-05MG human glioblastoma cells. The cells were exposed to 6 MV photon beam in single exposure with radiation doses of 0, 0.5, 1, 2 and 4 Gy. The RIBE were quantified by using irradiated cells’ culture media transferred to non-irradiated bystander cells 1 hour post-irradiation. Cells viability was measured using PrestoBlue assay after 24 and 48 hours. Meanwhile, the cell survivals of the bystander cells were determined using clonogenic assay. Cell viability of the bystander cells which received irradiated conditioned media from target cells and bystander cells which received media from sham-irradiated target cells shows no significant differences (p > 0.05). However, the cells survivals are found to decrease around 31.16% at dose of 4 Gy which suggest the presence of RIBE responses. The cell survival results demonstrated the possible RIBE responses in bystander cells which received medium transfer from irradiated cells. Our current data conclude that RIBE is important factors that need to be considered in radiation therapy.