ISOLATION AND OPTIMIZATION OF INTER-SIMPLE SEQUENCE REPEAT (ISSR) TECHNIQUE FOR PLEUROTUS SAJOR CAJU TOWARDS ENVIROMENTAL STUDY IN SUPPORT OF THE COUNTRY’S NUCLEAR POWER PROGRAMME
Mushroom can be used as a biological indicator in assessing radiological impact on the environment. Radiological effect would be reflected through morphological changes as well as those changes at molecular level. For this purpose, a preliminary work was conducted, which included DNA isolation, optimization of PCR parameters for Inter-Simple Sequence Repeat (ISSR) and primers screening on Pleurotus sajor caju mushroom strains from Nuclear Malaysia’s Sterifeed Mushrooms Collection Centre. In this work, DNA isolation technique from cap and stalk of fruit body were optimized and quantified. It was found that stalk produced highest amount of genomic DNA at 304.01ng/µl and cap at 149.00ng/µl. A total of 100 ISSR primers were tested and 51 primers were successfully amplified. These primers will be used further for dose response evaluation and molecular profiling in mushroom species.